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Bourne, Christina R

Associate Research Scientist
As of Jan. 1, 2014:
Assistant Professor, Dept. Chemistry & Biochemistry, University of Oklahoma

Specialty: Structural Biology and Biochemistry





Ph. D.

Department: Veterinary Pathobiology

Contact Information:
Veterinary Pathobiology
250 McElroy Hall
Stillwater, OK 74078
Office: (405) 744-6736
Send eMail

Biography:


2012 - present  Associate Research Scientist

2007 - present  Assistant Research Scientist

                         Oklahoma State University, Department of Veterinary Pathobiology

                         Laboratory of Dr. William Barrow



2005 - 2007  American Cancer Society Mary Horton Postdoctoral Fellow

                        University of Oklahoma Health Sciences Center, Department of Biochemistry and Molecular Biology

                        Research Focus: Anti-viral development of a Hepatitis B virus capsid protein assembly inhibitor

                        Mentor:  Dr. Adam Zlotnick


2003 - 2005  Postdoctoral Fellow

                        University of Oklahoma Health Sciences Center, Department of Biochemistry and Molecular Biology

                        Research Focus: Structural studies of the Hepatitis B virus capsid protein

                        Mentor:  Dr. Adam Zlotnick


1998 - 2003  Doctor of Philosophy

                       Oklahoma Medical Research Foundation, Department of Crystallography

                       University of Oklahoma Health Sciences Center, Department of Biochemistry and Molecular Biology

                       Dissertation:  Biochemical characterization and three-dimensional structures of the Fab and Fc

                       portions of a human IgG1 antibody

                       Mentor: Allen B. Edmundson, Ph.D.




Current Research Focus:


Abstract concepts make more sense with a vivid picture.  I pursue the visualization of molecules by application of crystallographic techniques.  The end results allows us to view the molecule in question, infer functional aspects, and if present in the experiment to understand how a small molecule binds and could affect the function.  I am currently using the same process in the development of new antibiotics, in addition to techniques including biochemistry and chemical biology.



Professional Experience:



2011-present    Member, BEI Resources Scientific Focus Group for Biodefense and High Containment bacteria


2011-present    Member, Advisory Board, Journal of Molecular Recognition


2010                  Guest scientist for Stillwater area “Born to do Science” science outreach program


2009                  MolSoft2009 Workshop on Modern Drug Target Crystallography and Structure Based Drug Discovery,

                                    San Diego, CA


2006-present    Ad Hoc reviewer: Journal of Antimicrobial Chemotherapy, Acta Crystallographica D,

                                  Acta Crystallographica F, Journal of Molecular Recognition


2000                  Participant in RapiData Biology Department, National Synchrotron Light Source,

                                 Brookhaven National Laboratory, Upton, NY


1999                  Oklahoma State University Mini-Course in Protein Purification, Stillwater, OK


1998                  Light Scattering University, Wyatt Technology, Santa Barbara, CA




Professional Affiliations:



2012-present  Member, International Chemical Biology Society


2008-present  Member, American Society for Microbiology


2006-2007       Member, American Society of Virology


2003-present  Member, American Society for Biochemistry and Molecular Biology


2001-present  Member, American Crystallographic Association


2001                President, Alpha Epsilon Lambda Honor Society (OUHSC branch)


1998-2006       Member, American Chemical Society




Selected Publications:



Bourne, C.R., Wakeham, N., Nammalwar, B., Tseitin, V., Bourne, P.C., Barrow, E.W., Ramnarayan, K., Bunce, R.A., Berlin, K.D., Barrow, W.W. (2013) Structure-activity relationships guide enantiomeric preference among potent inhibitors of B. anthracis dihydrofolate reductase. Biochemica et Biophysica Acta: Proteins and Proteomics. 1834:46-52. 


Nammalwar, B., Bunce, R. A., Berlin, K. D., Bourne, C. R., Bourne, P. C., Barrow, E. W., Barrow, W. W.  (2013) Heck synthesis of 2,4-diaminopyrimidine-based antibiotics using the Frech catalyst. Organic Preparationsand Procedures International. 45(1):66-71.


Nammalwar, B., Bourne, C.R., Bunce, R.A., Wakeham, N., Bourne, P.C., Ramnarayan, K., Mylvaganam, S., Berlin, K.D., Barrow, E.W., Barrow, W.W.  (2012)  6-Alkyl-2,4-diaminopyrimidine Inhibitors of Bacterial Dihydrofolate Reductase Can Open a Side Pocket Containing Conserved Waters.  ChemMedChem. 7:1974-82.     *Featured on inside cover


Barrow, E., Clinkenbeard, P., Duncan-Decocq, R., Perteet, R., Hill, K., Bourne, P.,  Valderas, M., Bourne, C., Clarkson, N., Clinkenbeard, K., Barrow, W.  (2012) High-throughput screening of a diversity collection using biodefense category A and B Priority pathogens.  Journal of Biomolecular Screening17(7):946-56. 


Nammalwar, B., Bunce, R.A., Berlin, K.D., Bourne, C.R., Bourne, P.C., Barrow, E.W., Barrow, W.W.  (2012) Synthesis and biological activity of substituted 2,4-diaminopyrimidines that inhibit Bacillus anthracis European Journal of Medicinal Chemistry 54:387-96. 


Nammalwar, B., Bunce, R. A., Berlin, K. D., Bourne, C. R., Bourne, P. C., Barrow, E. W., Barrow, W. W. (2012) Microwave-assisted Heck synthesis of substituted 2,4-diaminopyrimidine-based antibiotics. Organic Preparations and Procedures International. 44:281-87.


Bourne, C.R., Wakeham, N., Bunce, R.A., Nammalwar, B., Berlin, K.D., Barrow, W.W.  (2012) Classifying compound mechanism of action for linking whole cell phenotypes to molecular targets.  Journal of Molecular Recognition 25:216-23.


Bourne, C.R., Barrow, E.W., Bunce, R.A., Bourne, P.C., Berlin, K.D., Barrow, W.W.  (2010) Inhibition of antibiotic resistant Staphylococcus aureus by the broad-spectrum dihydrofolate reductase inhibitor RAB1.  Antimicrobial Agents and Chemotherapy 54(9): 3825-33.


Bunce, R.A., Bourne, C.R., Bourne, P.C., Barrow, E.B., Barrow, W.W., Berlin, K.D.  (2010) HPLC Detection and enantiomeric Separation of (+)- and (-)-(E)-3-[5-(2,4-diaminopyrimidin-5-ylmethyl)-2,3-dimethoxyphenyl]-1-(1-propyl-1h-phthalazin-2-yl)propenone, inhibitors of Bacillus anthracis dihydrofolate reductase, by supercritical fluid chromatography.  Proc. Of the Oklahoma Academy of Science 90:139-142.


C.R. Bourne, R.A. Bunce, P.C. Bourne, K.D. Berlin, E.W. Barrow, W.W. Barrow  (2009) Crystal structure of Bacillus anthracis dihydrofolate reductase with the dihydrophthalazine-based trimethoprim derivative RAB1 provides a structural explanation of potency and selectivity.  Antimicrobial Agents and Chemotherapy 53 (7): 3065-73.


C.R. Bourne, S.P. Katen, M.R. Fulz, C. Packianathan, A. Zlotnick (2009) A mutant Hepatitis B virus core protein mimics inhibitors of icosahedral capsid self-assembly.  Biochemistry 48 (8): 1736-42.


C. Bourne, S. Lee, B. Venkataiah, A. Lee, B. Korba, M.G. Finn, A. Zlotnick (2008) Small-molecule effectors of Hepatitis B virus capsid assembly give insight into virus lifecycle.  Journal of Virology 82: 10262-70.


A. Zlotnick, A. Lee, C.R. Bourne, J.M. Johnson, P.L. Domanico, S.J. Stray (2007) In vitro screening for molecules that affect virus capsid assembly and other protein association reactions.  Nature Protocols 2 (3): 490-98.  *Journal cover was figure for this manuscript


C.R. Bourne, M.G. Finn, A. Zlotnick (2006) Global structural changes in Hepatitis B capsids induced by the assembly effector HAP1.  Journal of Virology 80 (22): 11055-61.


P.A. Ramsland, S.S. Terzyan, G. Cloud, C.R. Bourne, W. Farrugia, G. Tribbick, M.H. Geysen, C.R. Moomaw, C.A. Slaughter, A.B. Edmundson (2006) Crystal structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody.  Biochemical Journal 395 (3): 473-81.


S.J. Stray, C.R. Bourne, S. Punna, W.G. Lewis, M.G. Finn, A. Zlotnick (2005) A heteroaryldihydropyrimidine activates and can misdirect Hepatitis B virus capsid assembly.  Proceedings of the National Academies of Science USA 102 (23):8138-40.






Courses Taught:


·  Guest Lecturer, VMED7114 Veterinary Physiology I, 4 hours contact, skeletal muscle (2013)

·  Guest Lecturer, VMED7114 Veterinary Physiology I, 4 hours contact, muscle biochemistry (2012)

·  Team teaching, BIOC6214 Physical Biochemistry of Macromolecules, 2 lectures, OUHSC (2007)

·  Team teaching, BIOC6321 Nuts and Bolts of Crystallography, 3 lectures, OUHSC (2007)

·  Adjunct Instructor, BIOC1514 Microbiology of Infectious Disease, Oklahoma City Community College, didactic and laboratory course, 8.5 contact hours per week (summer 2003)